Premium
Expression of molecular chaperones and vesicle transport proteins in differentiating oligodendrocytes
Author(s) -
Neri Christine L.,
Duchala Cynthia S.,
Macklin Wendy B.
Publication year - 1997
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19971201)50:5<769::aid-jnr13>3.0.co;2-x
Subject(s) - microbiology and biotechnology , chemistry , vesicle , biology , biochemistry , membrane
The major stages of oligodendrocyte differentiation are defined by the presence or absence of certain myelin‐specific proteins. Events leading to the successful processing of these proteins, such as the folding, assembly, and trafficking of these proteins through the biosynthetic pathway, are largely undefined. In the present study, we have examined both cultured primary oligodendrocytes and immortalized oligodendrocyte cell lines for the presence of molecular chaperones and/or vesicle transport proteins. We find that a select set of these proteins are expressed relatively early in oligodendrocyte differentiation, whereas a characteristically different set of proteins are expressed at later stages of oligodendrocyte differentiation. In other systems, these proteins participate in the folding and assembly of protein complexes, in the prevention of protein aggregation, as well as the trafficking of proteins via vesicles to specific subcellular destinations including the plasma membrane. Some of the chaperones and/or vesicle transport proteins investigated in this study may play a pivotal role in the certain aspect of myelin biogenesis. J. Neurosci. Res. 50:769–780, 1997. © 1997 Wiley‐Liss, Inc.