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Microglia and astroglia have a common progenitor cell
Author(s) -
Fedoroff Sergey,
Zhai Ruiling,
Novak Jaroslav P.
Publication year - 1997
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19971101)50:3<477::aid-jnr14>3.0.co;2-3
Subject(s) - microglia , progenitor cell , progenitor , neuroscience , biology , microbiology and biotechnology , stem cell , immunology , inflammation
Disaggregated neopallial cells from newborn C 3 H/HeJ mice were cloned in Grenier hybridoma tissue culture dishes, and culture wells that contained only one cell were marked. After 8–10 days of culturing, the cultures were fixed and double immunolabeled for microglia with Mac‐1 antibody and for astroglia with antibody to GFAP. Each marked well containing a clone was identified as either a microglia, astroglia, mixed microglia–astroglia, or an unlabeled clone. The effect of LM cell line conditioned medium (LM‐CM), which contains colony‐stimulating factor‐1, on the development of mixed microglia–astroglia clones was determined. Formation of mixed clones was dose dependent ( P < 0.0001). We concluded that microglia and astroglia have a common progenitor cell and that the development of mixed clones is LM‐CM dependent. J. Neurosci. Res. 50:477–486, 1997. © 1997 Wiley‐Liss, Inc.