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Short‐term response of postnatal rat vestibular neurons following brain‐derived neurotrophic factor or neurotrophin‐3 application
Author(s) -
Montcouquiol Mireille,
Valat Jean,
Travo Cécile,
Sans Alain
Publication year - 1997
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19971101)50:3<443::aid-jnr10>3.0.co;2-7
Subject(s) - neurotrophin , neurotrophin 3 , tropomyosin receptor kinase b , brain derived neurotrophic factor , scarpa's ganglion , neurotrophic factors , neuroscience , endocrinology , medicine , biology , tropomyosin receptor kinase c , calcium in biology , receptor , microbiology and biotechnology , vestibular system , intracellular , growth factor , platelet derived growth factor receptor
The effects of the application of brain‐derived neurotrophic factor (BDNF) and neurotrophin‐3 (NT‐3) neurotrophins on the intracellular calcium level ([Ca 2+ ] i ) were studied in vestibular ganglion neurons (VGNs) from postnatal day 3 (P3) rats cultured for 50 hr. We first assessed the expression of trkB and trkC mRNA receptors in cultured VGNs. Immunobloting and immunocytochemistry confirmed the presence of the neurotrophin receptors on neurons. Both neurotrophins induced transient [Ca 2+ ] i elevations in VGNs: BDNF‐treated neurons responded in 65% and NT‐3‐treated neurons in 56%. The responses could be inhibited by anti‐BDNF or anti‐NT‐3 antibodies. The [Ca 2+ ] i elevation was dependent on extracellular calcium since it was abolished in calcium‐free medium but also implicates the release of calcium from intracellular stores as tested by prior depletion with thapsigargin. Our results suggest the implication of a short‐term calcium regulation in VGNs, which could reflect specific fast effects of neurotrophins in the early postnatal rat vestibular system. J. Neurosci. Res. 50:443–449, 1997. © 1997 Wiley‐Liss, Inc.