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Developmental expression of nitric oxide synthase isoform I and III in chick retina
Author(s) -
Goureau Olivier,
RégnierRicard Fabienne,
Jonet Laurent,
Jeanny JeanClaude,
Courtois Yves,
ChanyFournier Françoise
Publication year - 1997
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19971001)50:1<104::aid-jnr11>3.0.co;2-b
Subject(s) - nitric oxide synthase , retina , nicotinamide adenine dinucleotide phosphate , citrulline , biology , microbiology and biotechnology , retinal , calretinin , nitric oxide , nicotinamide adenine dinucleotide , gene isoform , cytosol , biochemistry , neuroscience , enzyme , immunohistochemistry , arginine , endocrinology , nad+ kinase , amino acid , gene , oxidase test , immunology
During our studies on the multiple possible functions of nitric oxide (NO) in chick retinal development and physiology, we have demonstrated the presence and the activity of NO synthase (NOS‐I and III) in certain neuronal populations (photoreceptors, amacrine cells in the inner nuclear and ganglion cells) and also in synaptic‐rich regions in the developing chick retina. Both enzymes, detected by nicotinamide adenine dinucleotide phosphate (NADPH)‐diaphorase, immunohistochemistry and Western blotting, appeared between embryonic days 6 and 12, and followed a spatial and temporal pattern of expression which correlated with the differentiation of the neuronal layers. Evaluation of the conversion of [ 3 H]‐labeled arginine to [ 3 H]‐citrulline, confirmed the presence of a calcium‐dependent NOS activity in the cytosolic and particulate retinal extracts during the development. This pattern of NOS expression suggests that the regulated release of NO during key phases of development might be one mechanism involved in the regulation of retinal differentiation. J. Neurosci. Res. 50:104–113, 1997. © 1997 Wiley‐Liss, Inc.