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Thy‐1 and AvGp50 signal transduction complex in the avian nervous system: c‐Fyn and Gαi protein association and activation of signalling pathways
Author(s) -
Henke Robert C.,
Seeto Gregory S.,
Jeffrey Peter L.
Publication year - 1997
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19970915)49:6<655::aid-jnr1>3.0.co;2-6
Subject(s) - fyn , phosphoprotein , heterotrimeric g protein , signal transduction , biology , microbiology and biotechnology , g protein , phosphorylation , tyrosine kinase
We have previously reported the isolation of two distinct populations of detergent resistant membrane complexes (DRMC's) from day‐old chick brain (Henke et al.: J Neurosci Res 45:617–630, 1996). We now show that the glycosylphosphatidylinositol‐anchored proteins Thy‐1 and AvGp50 are associated in a signalling complex with c‐Fyn, the heterotrimeric Gαi subfamily members Gαi‐3, Gαz, and Gαo, α and β tubulin, and a number of other phosphoproteins in immunocomplexes isolated from both populations of DRMC's. Activation of this signalling complex via Thy‐1 monoclonal antibody incubation with chick forebrain cells, elicited a decrease in total phosphoprotein profile and tyrosine kinase activity present in DRMC fractions isolated from these cells, while AvGp50 and control antibodies had no effect. Down‐regulation of the DRMC phosphoprotein profile was accompanied by an increase in the Thy‐1‐associated signalling complex, suggesting that activation of this complex initiates the decreases seen in overall DRMC kinase activity. This signalling complex provides the basis for GPI‐anchored protein‐mediated signal transduction within the unique plasma membrane domains represented by DRMC's. J. Neurosci. Res. 49:655–670, 1997. © 1997 Wiley‐Liss, Inc.