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Multiple class I motifs revealed by sequencing naturally processed peptides eluted from rat T cell MHC molecules
Author(s) -
Burrows G.G.,
Ariail K.,
Celnik B.,
Gambee J.E.,
Offner H.,
Vandenbark A.A.
Publication year - 1997
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19970701)49:1<107::aid-jnr12>3.0.co;2-0
Subject(s) - mhc class i , elution , computational biology , major histocompatibility complex , class (philosophy) , biology , chemistry , genetics , antigen , computer science , chromatography , artificial intelligence
Class I major histocompatibility complex (MHC) molecules interact with a diverse array of self and foreign peptides. Displayed on the cell surface, the class I/peptide complex provides an extracellular indication of the intracellular milieu. We have characterized the Lewis rat Vβ8.2+ T cell hybridoma C14/BW12‐12A1 by FACS analysis and have used immuno‐affinity chromatography to purify class I molecules from these cells. Peptides eluted from the class I molecules have been fractionated by HPLC and sequenced. Self‐peptide mixtures indicate two distinct peptide motifs, suggesting the possibility of multiple class I loci. The majority of the naturally processed peptide ligands were nonamers. Naturally processed peptide ligands fitting the first motif contained a hydrophobic leucine anchor residue at position three and a carboxyl‐terminal serine anchor residue. A second motif was characterized by a tyrosine or phenylalanine residue at position three and a phenylalanine or isoleucine carboxyl‐terminal residue. Four peptides derived from the Vβ8.2 T cell receptor have sequences that fit these motifs, providing a mechanistic explanation for their immunoregulatory role. Identification of these class I peptide binding motifs will be useful for predicting potential CTL epitopes in studies on autoimmunity, immunoregulation and transplantation in the Lewis rat. J. Neurosci. Res. 49:107–116, 1997. © 1997 Wiley‐Liss Inc.