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Inducible nitric oxide synthase and nitric oxide production by oligodendrocytes
Author(s) -
Merrill Jean E.,
Murphy Sean P.,
Mitrovic Branislava,
MackenzieGraham Allan,
Dopp Joel C.,
Ding Minzhen,
Griscavage Jeannette,
Ignarro Louis J.,
Lowenstein Charles J.
Publication year - 1997
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19970515)48:4<372::aid-jnr9>3.0.co;2-8
Subject(s) - nitric oxide synthase , nitric oxide , western blot , microglia , microbiology and biotechnology , northern blot , in situ hybridization , calmodulin , astrocyte , in vitro , blot , oligodendrocyte , immunohistochemistry , messenger rna , chemistry , biology , enzyme , biochemistry , gene , inflammation , endocrinology , immunology , central nervous system , myelin
It has been previously demonstrated that microglia and astrocytes produce micromolar amounts of nitric oxide in vitro. In this study, we demonstrate that primary rat oligodendrocytes can be stimulated to produce iNOS mRNA as detected by Northern blot and in situ hybridization analysis and a 131‐kDa iNOS protein by Western blot analysis; protein was also detected in cells by single‐ and double‐label immunohistochemistry for iNOS and the oligodendrocyte‐specific marker CNPase. NO/NOS are produced as a consequence of activation of the gene encoding the inducible nitric oxide synthase as determined by inhibition with actinomycin D and cyclohexamide. The iNOS is functional, leading to calcium/calmodulin‐independent NO production in these in vitro cultures. J. Neurosci. Res. 48:372–384, 1997. © 1997 Wiley‐Liss, Inc.