Premium
Retinoic acid induced neural differentiation in a neuroectodermal cell line immortalized by p53 deficiency
Author(s) -
Schlett Katalin,
Madarász Emilia
Publication year - 1997
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19970215)47:4<405::aid-jnr6>3.0.co;2-i
Subject(s) - neuroepithelial cell , nestin , retinoic acid , progenitor cell , biology , embryonic stem cell , microbiology and biotechnology , neural cell , neural stem cell , precursor cell , cellular differentiation , forebrain , neural tube , cell culture , cell , neuroscience , embryo , stem cell , central nervous system , genetics , gene
Neuroepithelial cell lines were established from cerebral vesicles of 9‐day‐old mouse embryos lacking functional p53 genes (Livingstone et al: Cell 70:923–935, 1992). All‐trans retinoic acid (RA) induced bulk formation of neurons both in several p53‐deficient neuroepithelial cell lines and in wild‐type neural cells derived from early embryonic (E9–E12) forebrain vesicles. Forty‐eight‐hour treatment with 10 −6 MRA was necessary and sufficient to initiate neuron formation by p53 ‐/‐ ‐progenitors, but neuronal characteristics appeared with a delay of 3–4 days. The first appearance of cells with astroglial features followed that of neurons with a further delay of 4–5 days. The establishment of neuronal phenotypes involved minimally three rounds of cell cycle. Future neurons were sorted out from substrate‐attached cells and were characterized by a specific rearrangement of nestin‐immunoreactive filaments. The formation of neuronal phenotypes was not synchronized within the RA‐treated cell populations. The data indicate that RA, which promotes the initiation of neural differentiation, cannot function as a direct regulator of cell‐fate decisions made by neural progenitor cells. J. Neurosci. Res. 47:405–415, 1997. © 1997 Wiley‐Liss, Inc.