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Kainate/AMPA receptors expressed on human fetal astrocytes in long‐term culture
Author(s) -
Cauley Keith,
Kukekov Valery,
Young David
Publication year - 1997
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19970201)47:3<311::aid-jnr9>3.0.co;2-l
Subject(s) - kainate receptor , ampa receptor , glial fibrillary acidic protein , biology , microbiology and biotechnology , receptor , vimentin , astrocyte , population , nestin , glutamate receptor , neural stem cell , biochemistry , immunology , neuroscience , stem cell , central nervous system , immunohistochemistry , demography , sociology
Long‐term cultivation of primary human fetal brain cells has yielded a homogeneous population of glial progenitors of extended life span. These human astrocyte precursor (HAP‐1) cells have been in culture for greater than 1 year, are diploid, and do not form colonies in soft agar. The culture was established in 10% fetal calf serum (FCS), although cells greatly increase their proliferative rate when both basic fibroblast growth factor and FCS are present in the culture media. HAP‐1 cells express the cytoskeletal proteins glial fibrillary acidic protein, vimentin, and nestin. HAP‐1 cells express the AMPA/kainate receptor subunit genes GluRs 1, 3, and 4 and the kainate receptor subunit genes GluR6, KA1, and KA2. Immunohistochemistry confirms the expression of GluR subunit proteins. HAP‐1 cells demonstrate a kainate‐responsive current found to be blockable by CNQX. HAP‐1 cells will serve in the study of human glial cells and ligand‐gated ion channels and in the identification of compounds which might act as agonists or antagonists at these receptor‐ion channel complexes. J. Neurosci. Res. 47:311–321, 1997. © 1997 Wiley‐Liss, Inc.