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Ultrastructural localization of 5‐hydroxytryptamine 1A receptors in the rat brain
Author(s) -
Kia Hossein K.,
Brisorgueil MarieJeanne,
Hamon Michel,
Calas André,
Vergé Daniel
Publication year - 1996
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19961215)46:6<697::aid-jnr7>3.0.co;2-a
Subject(s) - dorsal raphe nucleus , immunolabeling , raphe nuclei , nucleus , biology , receptor , cytoplasm , neuroscience , dendritic spine , hippocampus , serotonergic cell groups , median raphe nucleus , microbiology and biotechnology , anatomy , serotonin , serotonergic , immunohistochemistry , hippocampal formation , immunology , biochemistry
Abstract 5‐Hydroxytryptamine 1A (5‐HT 1A ) receptors have been visualized at the electron microscopic level in selected areas (dorsal raphe nucleus, hippocampus, septum) of the rat brain using specific anti‐peptide antibodies. 5‐HT 1A receptor immunoreactivity was found almost exclusively in the somatodendritic compartment of neurons and was very rarely observed within processes possibly belonging to glial cells. The immunoenzymatic reaction product was associated exclusively with dendritic spines in the dorsal hippocampus, whereas in the dorsal raphe nucleus and the septal complex, immunoreactivity was found in both dendritic processes and somata. Although some immunolabeling was observed within the cytoplasm of cell bodies, 5‐HT 1A receptor immunoreactivity was essentially confined to the plasma membrane where it was unevenly distributed. It was frequently associated with synapses (except in the dorsal raphe nucleus), but was also found extrasynaptically in both somata and dendrites. These data suggest that the action of serotonin via 5‐HT 1A receptor could occur through junctional as well as nonjunctional transmission. © 1996 Wiley‐Liss, Inc.