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Endothelin‐3 attenuates the cyclic GMP responses to C‐type natriuretic peptide in cultured mouse astrocytes
Author(s) -
Yeung V.T.F.,
Ho S.K.S.,
Tsang D.S.C.,
Nicholls M.G.,
Cockram C.S.
Publication year - 1996
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19961215)46:6<686::aid-jnr6>3.0.co;2-b
Subject(s) - staurosporine , protein kinase c , medicine , cycloheximide , endocrinology , thapsigargin , second messenger system , wortmannin , okadaic acid , protein kinase a , natriuretic peptide , chemistry , biology , signal transduction , receptor , kinase , calcium , biochemistry , phosphatase , phosphatidylinositol , protein biosynthesis , enzyme , heart failure
The effect of endothelin‐3 (ET‐3) on cyclic GMP (cGMP) responses to C‐type natriuretic peptide (CNP) was studied in primary cultures of mouse astrocytes. Attenuation of CNP‐stimulated cGMP formation by ET‐3 was time‐dependent, with maximum inhibition achieved at 30 min of preincubation. ET‐3 suppressed cGMP production in response to 10 nM CNP in a dose‐dependent fashion, with an IC 50 of 0.04 nM and a maximal inhibitory concentration of 1 μM, which led to a 66% reduction of the cGMP increment from 45.0 ± 4.2 pmol/mg protein to 15.4 ± 2.6 pmol/mg protein. ET‐1, ET‐2, and ET‐3 were equipotent in suppressing the CNP‐induced cGMP response, suggesting that this effect was mediated by ET B receptors. Staurosporine, Ro 31‐8220, calcium‐free medium, nifedipine, verapamil, lanthanum, thapsigargin, BAPTA, W7, calmidazolium, U‐73122, neomycin, quinacrine, wortmannin, herbimycin‐A, okadaic acid, and sodium orthovanadate failed to block the effect of ET‐3. Cycloheximide (100 μM), however, partially but significantly reversed the inhibitory effect of ET‐3 on CNP‐induced cGMP from 48.2 to 73.3% of the control value. The results support the premise that ET‐3 and CNP interact within the central nervous system. The data also suggest that the inhibitory effect of ET‐3 on CNP‐stimulated cGMP accumulation in mouse astrocytes is mediated by activation of certain kinases through as yet undefined mechanisms and not by protein kinase C, increased intracellular calcium, or other second messenger pathways such as phospholipases A 2 , C, D, tyrosine kinase, or protein phosphatases. © 1996 Wiley‐Liss, Inc.