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Signaling effects of α‐thrombin and SFLLRN in rat glioma C 6 cells
Author(s) -
Kaufmann R.,
Lindschau C.,
Höer A.,
Henklein P.,
Adomeit A.,
Haller H.,
Liebmann C.,
Oberdisse E.,
Nowak G.
Publication year - 1996
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19961215)46:6<641::aid-jnr1>3.0.co;2-f
Subject(s) - thrombin , thrombin receptor , protein kinase c , neurite , glioma , inositol , microbiology and biotechnology , inositol phosphate , receptor , astrocyte , signal transduction , stimulation , biology , chemistry , biochemistry , platelet , endocrinology , cancer research , immunology , in vitro , central nervous system
Effects of thrombin on brain cells, including change of neurite outgrowth and astrocyte shape, are described, but the molecular mechanisms are unclear. We investigated the effects of human α‐thrombin and a six amino acid thrombin receptor activating peptide (TRAP‐6, SFLLRN) on [Ca 2+ ] 1 , phosphoinositide hydrolysis, and protein kinase C in rat glioma C 6 cells. Stimulation of C 6 cells with both α‐thrombin and TRAP‐6 resulted in [Ca 2+ ] 1 mobilization, [ 3 H]Inositol phosphate response, and enhanced immunoreactivity of the protein kinase C (PKC) isoenzymes α, β, γ, δ, and ϵ. Results suggest that α‐thrombin and TRAP‐6 activate at least partially the same intracellular signaling pathways in rat glioma C 6 cells, which is evidence for involvement of “tethered ligand” receptor in thrombin induced signaling in glioma C 6 cells. © 1996 Wiley‐Liss, Inc.