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Structural determinants of Trk receptor specificities using BNDF‐based neurotrophin chimeras
Author(s) -
Lai KwokOn,
Glass David J.,
Geis David,
Yancopoulos George D.,
Ip Nancy Y.
Publication year - 1996
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19961201)46:5<618::aid-jnr10>3.0.co;2-t
Subject(s) - trk receptor , tropomyosin receptor kinase a , tropomyosin receptor kinase b , neurotrophin , low affinity nerve growth factor receptor , tropomyosin receptor kinase c , biology , receptor , microbiology and biotechnology , nerve growth factor , neurotrophic factors , neuroscience , genetics , platelet derived growth factor receptor , growth factor
Neurotrophins play very important roles in the development and maintenance of the vertebrate nervous system. In mammals, there are four members of the family: NGF, BDNF, NT‐3, and NT‐4/5. Members of the neurotrophin family activate different receptors that belong to a class of receptor tyrosine kinases known as “Trks.” For example, NGF is the specific ligand of TrkA, while BDNF activates TrkB. To elucidate which regions of the two neurotrophins determine the receptor specificities, chimeric neurotrophins were constructed using BDNF as the backbone, with various regions being substituted by the corresponding regions of NGF. The activity of the chimeras on the Trk receptors was assayed in transfected fibroblasts ectopically expressing the Trk receptors. Our findings revealed that, although BDNF is absolutely conserved in mammals, substitution of several small variable regions from NGF into the BDNF backbone did not lead to significant loss in TrkB activity or gain in TrkA activity. Moreover, important determinants of TrkB activation might be located in the carboxy‐terminal half of BDNF. On the other hand, critical elements for TrkA activation might be located within the amino‐terminal half of the mature NGF molecule. © 1996 Wiley‐Liss, Inc.