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Expression of the neurofibromatosis 2 tumor suppressor gene product, merlin, in Schwann cells
Author(s) -
Scherer Steven S.,
Gutmann David H.
Publication year - 1996
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19961201)46:5<595::aid-jnr8>3.0.co;2-e
Subject(s) - merlin (protein) , schwann cell , microbiology and biotechnology , biology , neurofibromatosis , tumor suppressor gene , cytoskeleton , cell , suppressor , gene , genetics , carcinogenesis
The neurofibromatosis 2 ( NF2 ) tumor suppressor gene encodes a protein with sequence similarity to a family of molecules linking cell membrane proteins to the actin cytoskeleton. Individuals affected with NF2 develop schwannomas at an increased frequency. In this paper, we report that merlin is expressed in Schwann cells, where it is localized in vitro to the cell membrane by immunohista chemistry and subcellular fractionation. Exogenous expression of merlin fragments confirms this subcellular distribution and suggests that both the N‐terminal and C‐terminal portions of the molecule are required for this localization. In addition, merlin is expressed in rat sciatic nerve Schwann cells at paranodal membranes, where it colocalizes with RhoA. Lastly, expression of the NF2 gene increases during postnatal rat sciatic nerve development, consistent with its role as a negative growth regulator for Schwann cells. These results collectively suggest that merlin may function at the cell surface to modulate cell growth in Schwann cells and to link cell membrane proteins to the cytoskeleton. © 1996 Wiley‐Liss, Inc.