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Intracellular pH regulation in cultured microglial cells from mouse brain
Author(s) -
Faff L.,
Ohlemeyer C.,
Kettenmann H.
Publication year - 1996
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19961101)46:3<294::aid-jnr2>3.0.co;2-f
Subject(s) - intracellular , microbiology and biotechnology , microglia , neuroscience , chemistry , biology , immunology , inflammation
Intracellular pH (pH 1 ) and the mechanisms of pH 1 regulation have been investigated in cultured microglial cells from mouse brain using the pH‐sensitive fluorescent dye 2′,7′‐bis‐(2‐carboxyethyl)‐5‐(6)‐carboxyfluorescein (BCECF). Cells were acidified by a pulse of NH 4 + (4‐5 min; 20 mM) and the subsequent pH 1 recovery from an acidification was studied. In HCO 3 ‐ ‐free saline, pH regulation was dependent on extracellular [Na + ] and sensitive to amiloride, indicating the involvement of the Na + /H + exchanger. In HCO 3 ‐ ‐containing solution 2 mM amiloride slowed but did not block pH 1 recovery; the recovery however was dependent on extracellular [Na + ] and sensitive to 0.3 mM DIDS, suggesting the presence of Na + /HCO 3 cotransporter and/or Na + ‐dependent Cl ‐ /HCO 3 ‐ exchanger. The involvement of a Na‐dependent Cl ‐ /HCO 3 ‐ exchanger was inferred from the observation that removal of Cl ‐ or application of 1 mM furosemide decreased but did not block the recovery rate. Increasing [K + ] 0 resulted in an alkalinization by a process that was neither HCO 3 ‐ nor Na + ‐dependent, nor DIDS‐ and amiloride‐inhibitable. In conclusion, microglial cells express a distinct set of pH regulatory carriers which control for a defined level of pH 1 . An increase in [K + ] 0 can offset this level. © 1996 Wiley‐Liss, Inc.