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Effects of phenylsuccinate on potassium‐stimulated taurine release in cultured neurons and astrocytes and in rat hippocampus in vivo
Author(s) -
Bruhn Torben,
Christensen Thomas,
Cobo Manuela,
Damgaard Inge,
Diemer Nils H.,
Schousboe Arne
Publication year - 1996
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19961015)46:2<198::aid-jnr7>3.0.co;2-c
Subject(s) - taurine , microdialysis , in vivo , glutamate receptor , neurotransmitter , extracellular , hippocampus , in vitro , neuroglia , microbiology and biotechnology , chemistry , biology , biochemistry , central nervous system , neuroscience , amino acid , receptor
Swelling‐induced release of taurine was investigated in vivo in hippocampus by microdialysis or in vitro in cultured neocortical neurons or astrocytes. Swelling was induced either by increasing the extracellular K + concentration or by exposing the cells to hyposmotic conditions. It was found that the drug phenylsuccinate, which inhibits the mitochondrial dicarboxylate carrier as well as biosynthesis of neurotransmitter glutamate, inhibits swelling‐induced taurine release both in vivo and in cultured cells. Thus, phenylsuccinate may be used to investigate the mechanism involved in taurine release associated with regulatory volume decrease in cells. © 1996 Wiley‐Liss, Inc.