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Possible mechanism for the TCRβ‐chain associated EAE resistance of LER rats
Author(s) -
Bourque M.M.,
Martin A.M.,
DesquennesClark L.,
HeberKatz E.,
Blankenhorn E.P.
Publication year - 1996
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19960915)45:6<714::aid-jnr8>3.0.co;2-b
Subject(s) - t cell receptor , myelin basic protein , biology , t cell , beta (programming language) , immunology , experimental autoimmune encephalomyelitis , microbiology and biotechnology , polyclonal antibodies , encephalomyelitis , antigen , immune system , myelin , multiple sclerosis , neuroscience , computer science , programming language , central nervous system
LER rats are resistant to the active induction of experimental allergic encephalomyelitis (EAE). The mechanism of their resistance to EAE has yet to be defined, although LER rats are susceptible to adoptively transferred EAE. Genetic analysis of LER and the susceptible LEW rat suggests that a gene linked to the T cell receptor (TCR) β‐chain complex contributes to EAE resistance. This result is consistent with the fact that EAE is a T cell mediated disease and one characterized in EAE‐susceptible animals by an oligoclonal TCR Vβ8.2 + response. In this report, analysis of TCR transcripts by reverse transcriptase polymerase chain reaction (RT‐PCR) and restriction digestion demonstrates that LER lymph nodes, collected on day 10 post‐immunization with myelin basic protein (MBP), express both TCR‐Vβ8.2 and other TCRβ chains, usually Vβ8.4, whereas LEW animals demonstrate preferential and almost exclusive use of Vβ8.2 TCR. Fluorescence‐activated cell sorting (FACS) analyses of anti‐MBP T cells confirm that LER T cells express Vβ8.2 TCR to a lesser degree than LEW T cells. Finally, experiments examining the oligo‐ or polyclonality of the TCRVβ CDR3 region show that the LER response to MBP is polyclonal, while the LEW response to MBP is oligoclonal. Therefore, the cumulative data on the TCR usage profiles in this report suggest that the choice of TCR variable β‐chain may contribute to the resistance seen in the LER rat. © 1996 Wiley‐Liss, Inc.