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Activation of the gene encoding decay accelerating factor following nerve growth factor treatment of sensory neurons is mediated by promoter sequences within 206 bases of the transcriptional start site
Author(s) -
Kendall G.,
Crankson H.,
Ensor E.,
Lublin D.M.,
Latchman D.S.
Publication year - 1996
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19960715)45:2<96::aid-jnr2>3.0.co;2-a
Subject(s) - nerve growth factor , dorsal root ganglion , gene , biology , transfection , microbiology and biotechnology , messenger rna , gene expression , neuroscience , sensory system , genetics , receptor
Using two independent differential screening procedures designed to identify novel mRNAs induced by nerve growth factor (NGF) treatment of adult dorsal root ganglion (DRG) neurons, we have isolated cDNA clones derived from the gene encoding decay accelerating factor (DAF). Hybridization analysis and semi‐quantitative polymerase chain reaction confirmed that the DAF mRNA was indeed induced in NGF‐treated adult DRG neurons. Moreover, the DAF gene promoter is NGF inducible (approximately two‐ to threefold) when transfected into DRG neurons, and this effect is primarily dependent on sequences between −206 and −77 relative to the transcriptional start site. Hence, the DAF gene constitutes a novel NGF‐inducible gene whose mRNA is elevated in response to NGF treatment of DRG neurons. The potential significance of this effect is discussed in terms of the role of NGF in modulating the transcriptional activity and function of adult DRG neurons. © 1996 Wiley‐Liss, Inc.