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Presence of calbindin D 28K and GAD 67 mRNAs in both orthotopic and ectopic purkinje cells of Staggerer mice suggests that Staggerer acts after the onset of cytodifferentiation
Author(s) -
Frantz G.D.,
Wuenschell C.W.,
Messer A.,
Tobin A.J.
Publication year - 1996
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19960501)44:3<255::aid-jnr6>3.0.co;2-f
Subject(s) - in situ hybridization , biology , calbindin , ectopic expression , purkinje cell , microbiology and biotechnology , gene expression , embryonic stem cell , mutant , gene , cerebellum , endocrinology , immunohistochemistry , genetics , immunology
We used in situ hybridization to study the expression of GAD 67 and calbindin D 28K mRNAs in developing mouse cerebellar Purkinje cells. Both genes are expressed prenatally; calbindin D 28K mRNAs can be detected in Purkinje cells of embryonic day (E) 15 mice, whereas GAD 67 mRNAs first appear slightly later, in E16 mice. The stunted Purkinje cells of staggerer (sg/sg) mutant mice maintain calbindin D 28K and GAD 67 expression. Our data suggest that the sg/sg mutation does not interfere with the transcriptional activation of these two genes, and might therefore act after the induction of specific gene expression in developing Purkinje cells. © 1996 Wiley‐Liss, Inc.