Premium
Transforming growth factor‐beta 3, glial cell line‐derived neurotrophic factor, and fibroblast growth factor‐2, act in different manners to promote motoneuron survival in vitro
Author(s) -
Gouin A.,
BlochGallego E.,
Tanaka H.,
Rosenthal A.,
Henderson C. E.
Publication year - 1996
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19960215)43:4<454::aid-jnr6>3.0.co;2-e
Subject(s) - glial cell line derived neurotrophic factor , neurotrophic factors , fibroblast growth factor , gdnf family of ligands , neurotrophin , biology , transforming growth factor , transforming growth factor beta , population , basic fibroblast growth factor , neuroscience , microbiology and biotechnology , embryonic stem cell , nerve growth factor , growth factor , cell culture , medicine , genetics , receptor , environmental health , gene
Developing chick motoneurons depend on as yet unidentified factors from the periphery and the central nervous system for their survival. Using cultures of purified embryonic motoneurons, we show that basic fibroblast growth factor (FGF‐2) or transforming growth factor‐beta 3 (TGFβ3) each have only low survival‐promoting activity when tested alone, but act synergistically to keep motoneurons alive for at least 3 days. Glial cell line‐derived neurotrophic factor (GDNF), another member of the TGFβ family, was itself sufficient to maintain a population of motoneurons. However, its effect was not significantly increased by the addition of FGF‐2. These results suggest that FGF‐2, TGFβ3, and GDNF, which are all present in the environment of developing motoneurons, may act by different mechanisms as physiological survival factors for this population of central neurons. © 1996 Wiley‐Liss, Inc.