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High expression of Bcl‐x protein in the developing human cerebellar cortex
Author(s) -
Sohma O.,
Mizuguchi M.,
Takashima S.,
Yamada M.,
Ikeda K.,
Ohta S.
Publication year - 1996
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19960115)43:2<175::aid-jnr5>3.0.co;2-d
Subject(s) - cerebellar cortex , neuroscience , cortex (anatomy) , expression (computer science) , cerebellum , protein expression , human brain , microbiology and biotechnology , biology , computer science , biochemistry , gene , programming language
The gene bcl‐x, which is related to a bcl‐2, regulates programmed cell death. bcl‐x may function in the development of the nervous system. We raised a polyclonal antibody against human Bcl‐x protein, and investigated its distribution in the developing human cerebellum. Western blotting revealed that Bcl‐x expression in the cerebellum is higher in the fetal, than in the postnatal period. Immunohistochemical studies of fetal brains localized intense Bcl‐x immunoreactivity in the granule cell processes at 13–22 gestational weeks and in the Purkinje cell bodies at 24–38 weeks. The immunoreactivity decreased after birth, but was retained in the Purkinje cells at a low level until adulthood. These results suggested that Bcl‐x expression in the cerebellum is developmentally regulated and involved specifically in the development of neuronal subpopulations. © 1996 Wiley‐Liss, Inc.