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Comparison between proton‐induced x‐ray emission (PIXE) and total reflection x‐ray fluorescence (TXRF) spectrometry for the elemental analysis of human amniotic fluid
Author(s) -
Liendo J. A.,
González A. C.,
Castelli C.,
Gómez J.,
Jiménez J.,
Marcó L.,
SajoBohus L.,
Greaves E. D.,
Fletcher N. R.,
Bauman S.
Publication year - 1999
Publication title -
x‐ray spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.447
H-Index - 45
eISSN - 1097-4539
pISSN - 0049-8246
DOI - 10.1002/(sici)1097-4539(199901/02)28:1<3::aid-xrs298>3.0.co;2-5
Subject(s) - x ray fluorescence , analytical chemistry (journal) , x ray , proton , chemistry , fluorescence , mass spectrometry , amniotic fluid , radiochemistry , materials science , chromatography , optics , pregnancy , fetus , physics , quantum mechanics , biology , genetics
A comparison between use of proton‐induced x‐rayemission (PIXE) and total reflection x‐rayfluorescence (TXRF) techniques in relation to the elementalanalysis of amniotic fluid (AF) was carried out bymeasuring the concentrations of Cl, K, Ca, Fe, Cu, Zn and Br. For 10AF samples analyzed, the agreement between PIXE and a TXRFmeasurements was 100% for K, 60% for Cl and Ca,80% for Fe and 50% for Cu. For Zn, although there wasonly a 20% agreement, the measured concentrations rangedbetween 0.1 and 1 μg/ml with a TXRF standard deviation as highas 0.070 μg/ml. For Br, a factor of 1.8±0.2 wasobtained between TXRF and PIXE measurements, suggesting a systematicerror between experiments. This work indicates that furtherinvestigation is required to determine the experimental conditions,e.g. sample preparation and irradiation, which lead to completeagreement between TXRF and PIXE when a complicated organic samplesuch as amniotic fluid is analyzed. Copyright © 1999 John Wiley& Sons, Ltd.