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Semiquantitative analysis of the cellular preservation quality of Normosol® and Carbowax® solutions for thyroid fine‐needle aspiration specimens
Author(s) -
Nasuti Joseph F.,
Fleisher Seth R.,
Cobbs B. Charlene,
Gupta Prabodh K.
Publication year - 2000
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/(sici)1097-0339(200005)22:5<319::aid-dc12>3.0.co;2-7
Subject(s) - medicine , fine needle aspiration , fixative , thyroidectomy , thyroid , nuclear medicine , surgery , pathology , biopsy , frozen section procedure
The present study was designed to compare cytomorphologic features and diagnostic value of thyroid fine‐needle aspiration (FNA) specimens stored in Saccomano's (Carbowax® solution) fixative and Normosol® solutions. Equivalent amounts of aspirate material obtained from nonlesional areas of 17 freshly resected thyroidectomy specimens were placed in room temperature Carbowax® solution and refrigerated Normosol® solution for a time period of up to 60 hr. Millipore® filter preparations were made from both fluids for each of the 17 thyroidectomy specimens. Slides were examined by three independent observers who rated the quality of cellular preservation based on a four‐part semiquantitative scale. Statistical analysis of the data obtained from the scores of the three observers showed no statistically significant difference in the quality of specimen preservation. Normosol® preserved specimens consistently rated superior to Carbowax® in nuclear detail, lack of obscuring background artifact, and diagnostic value. These findings, along with a considerably less cost, make refrigerated Normosol® an attractive alternative for the short‐term storage of FNA specimens placed in fluid. Diagn. Cytopathol. 2000;22:319–322. © 2000 Wiley‐Liss, Inc.