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Polymerase chain reaction amplification of trichomonas vaginalis DNA from papanicolaou‐stained smears
Author(s) -
Okuyama Takazo,
Takahashi Rei,
Mori Miyuki,
Osaka Mitsuhiko,
Kobayashi Tadao K.,
Maeda Sakann
Publication year - 1998
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/(sici)1097-0339(199812)19:6<437::aid-dc6>3.0.co;2-a
Subject(s) - papanicolaou stain , trichomonas vaginalis , polymerase chain reaction , medicine , dna , cytopathology , pathology , cytology , biology , cervical cancer , gene , gynecology , cancer , genetics , biochemistry
DNA from Papanicolaou‐stained smears was successfully amplified using the polymerase chain reaction (PCR) to investigate if it could be used for retrospective genome studies, such as for the detection of Trichomonas vaginalis. DNA was isolated from 20 archival Papanicolaou‐stained smears (19 cervical and 1 seminal fluid samples) and purified by treatment with proteinase K, phenol/chloroform, and ethanol. The method of recovering DNA from the Papanicolaou‐stained smear is very important in the successful amplification of Trichomonas DNA in archival cytological smears. We used a specific sequence and successfully amplified DNA in cytological smears of T. vaginalis using PCR. We conclude that the diagnostic accuracy of the detection of T. vaginalis in cervical smears is enhanced by using a combination of a Papanicolaou‐stained smear and PCR. Diagn. Cytopathol. 1998;19:437– 440. © 1998 Wiley‐Liss, Inc.