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Performance optimization of the ThinPrep® processor: Effect of microscope slides
Author(s) -
Lapen Daniel C.,
Papillo Jacalyn
Publication year - 1998
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/(sici)1097-0339(199811)19:5<388::aid-dc17>3.0.co;2-2
Subject(s) - microscope , microscope slide , medicine , optical microscope , stain , inverted microscope , biomedical engineering , microscopy , scanning electron microscope , pathology , staining , materials science , composite material
Our objective was to evaluate the cellular adherence property of various microscope slides. Slide preparations were made using the ThinPrep ® 2000 System, in which cells are transferred from a filter to a slide. A total of eight slide types was selected for testing against the ThinPrep ® microscope slide: S/P MicroSlide, S/P Pusher, S/P Colorfrost, Menzel Superfrost, Sayag, Sail Brand, Fisher Plus, and Newcomer Poly‐L‐Lysine. Gynecologic samples from 4 different patients were utilized on the ThinPrep ® Processor. Multiple preparations were made from each sample using the different microscope slides. The slides were stained with a commercial Pap stain, and cellular material was quantified by scanning each preparation using a linear stage mounted on a Hitachi U‐2000 spectrophotometer set at 580 nm. The data generated by this study show that ThinPrep microscope slides produce preparations with the highest level of cellular material. This study quantifies slide performance, examines a range of microscope slide types, and shows that ThinPrep microscope slides exhibit significantly increased cell adherence over the other slide types tested. Diagn. Cytopathol. 1998;19:388–391. © 1998 Wiley‐Liss, Inc.