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Cytological aspiration of intraocular retinoblastoma in an 11‐year‐old boy
Author(s) -
Decaussin Myriam,
Boran Mojgan DevouassouxShishe,
Salle Monique,
Grange JeanDaniel,
Patricot LouisMarc,
ThivoletBejui Françoise
Publication year - 1998
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/(sici)1097-0339(199809)19:3<190::aid-dc7>3.0.co;2-h
Subject(s) - medicine , retinoblastoma , enucleation , pathology , fine needle aspiration , h&e stain , cytopathology , enolase , cytology , eye neoplasm , staining , biopsy , immunohistochemistry , surgery , biochemistry , chemistry , gene
Only a few cases of retinoblastomas in older children have been reported and the clinical diagnosis may be difficult. In case, fine‐needle aspiration from an atypical retinal mass of an 11‐yr‐old boy was performed. The vitreous fluid was stained with Diff‐Quik for an immediate cytological examination and the diagnosis of retinoblastoma was suggested. The rest of the specimen was separated into two parts. One was stained with May‐Grünwald‐Giemsa and the other was centrifuged, embedded in paraffin, and finally stained with hematoxylin‐eosin‐safran. The undifferentiated blue cells were associated with abundant necrotic debris and portions of capillaries with perivascular tumor cells around. The cytoplasm of the tumor cells was strongly stained with neuron‐specific enolase antibody. The diagnosis of retinoblastoma was confirmed. The specimen of enucleation confirmed the diagnosis. In conclusion, cytological aspiration can categorically diagnose suspected intraocular tumors of older children in whom clinical and noninvasive investigations have failed to establish the diagnosis. Diagn. Cytopathol. 1998;19:190–193. © 1998 Wiley‐Liss, Inc.