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Immunocytochemistry controls using cell culture
Author(s) -
Kurtycz Daniel F.I.,
Logrono Roberto,
Leopando Mark,
Slattery Anne,
Inhorn Stanley L.
Publication year - 1997
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/(sici)1097-0339(199707)17:1<74::aid-dc17>3.0.co;2-7
Subject(s) - immunocytochemistry , immunoperoxidase , cytokeratin , pathology , cell culture , medicine , antigen , cell , antibody , monoclonal antibody , immunohistochemistry , biology , immunology , biochemistry , genetics
A continuing problem in cytology laboratories is the lack of adequate control material for immunocytochemical testing. Usual control procedures involve testing paraffin‐embedded control materials along with the patient specimens. These control materials are fundamentally unlike cytologic preparations. We have developed a method to make control preparations for immunocytochemical analysis using cultured anaplastic cells with known antigenic features from commercial sources. Cell lines included melanoma, rhabdomyosarcoma, T‐cell leukemia, and squamous‐cell carcinoma. Modified Saccomano and acetone fixation coupled with the cytospin technique enabled good‐quality preparations. Cell lines were tested with antibodies for HMB‐45, actin, leukocyte common antigen (LCA), and cytokeratin, with avidin‐biotin immunoperoxidase and diaminobenzidine (DAB) as chromogens. Our final preparations were easily interpretable with excellent morphologic preservation of cellular detail. Cultured cells provide a superior method for preparing almost unlimited numbers of control slides for immunocytochemistry for laboratories with access to a tissue culture facility. Diagn. Cytopathol. 17:74–79, 1997. © 1997 Wiley‐Liss, Inc.