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In situ hybridization of chromosome 6 on fine‐needle aspirates from breast carcinomas: Comparison of numerical abnormalities and ER/PgR status and staining pattern
Author(s) -
Sauer Torill,
Beraki Kahsai,
Jebsen Peter Wilhelm,
Ormerod Eli,
Naess Oddvar
Publication year - 1997
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/(sici)1097-0339(199705)16:5<420::aid-dc7>3.0.co;2-f
Subject(s) - estrogen receptor , pathology , staining , progesterone receptor , chromosome , biology , aneuploidy , immunohistochemistry , breast cancer , medicine , cancer , gene , genetics
The estrogen receptor (ER) gene is located on chromosome 6. The aim of our study was to investigate whether numerical chromosomal aberrations were reflected in estrogen/progesterone receptor (PgR) status and staining pattern. Fine‐needle aspirates from 51 breast carcinomas were investigated immunocytochemically for ER/PgR and by in situ hybridization technique using digoxigenin‐labeled α‐satellite probe for chromosome 6. Cases with ≥70% two‐signal nuclei were regarded as disome; the remaining tumors showed aneusomy with a variable number of signals. Aneusomy was found in 32 tumors (63%), whereas 19 (37%) had a normal number of chromosome 6. Chromosomal gain occurred in all aneusome cases except one. ER‐ and/or PgR‐positive tumors had an equal distribution of disomy and aneusmy. Variable ER staining pattern or ER and/or PgR negativity was associated with numerical aberrations in chromosome 6 in 76% of the tumors. Cancers with uniform ER staining pattern all had normal chromosome number. Diagn. Cytopathol. 16:420–424, 1997. © 1997 Wiley‐Liss, Inc.