Open AccessTheoretical investigation of aspects of single‐molecule fluorescence detection in microcapillaries
Author(s) -
Enderlein Jörg
Publication year - 1999
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/(sici)1097-0320(19990701)36:3<195::aid-cyto8>3.0.co;2-1
Subject(s) - fluorescence , laser induced fluorescence , molecule , laser , scattering , optics , laser beams , capillary action , light scattering , materials science , chemistry , physics , organic chemistry , composite material
In the present report, the results of a theoretical investigation of two aspects of single‐molecule detection by laser‐induced fluorescence in microcapillaries are presented. The two issues studied are the scattering of the exciting laser beam on the microcapillary and the change of the fluorescence lifetime of the molecule due to the electrodynamic interaction between its fluorescence emission and the confining capillary. Numerical results for experimentally relevant conditions are provided. Cytometry 36:195–199, 1999. © 1999 Wiley‐Liss, Inc.