Open AccessRole of DNA aneuploidy, overexpression of p53 gene product, and cellular proliferation in the progression of gastric cancer
Author(s) -
Sugai Tamotsu,
Nakamura Shinichi,
Uesugi Noriyuki,
Habano Wataru,
Yoshida Toru,
Tazawa Hideki,
Orii Seishi,
Suto Takayuki,
Itoh Chuichi
Publication year - 1999
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/(sici)1097-0320(19990615)38:3<111::aid-cyto4>3.0.co;2-t
Subject(s) - aneuploidy , cancer , carcinogenesis , biology , cancer research , pathology , gene , medicine , genetics , chromosome
DNA aneuploidy, p53 overexpression, and high cell proliferation frequently occur in gastric cancer. However, little is known about the time of their appearance throughout cancer progression. Therefore, the objective of the present study was to determine when such abnormalities occur during gastric cancer progression. We classified the gastric cancers examined into intestinal (n = 65) and diffuse (n = 34) types. DNA ploidy was examined using flow cytometry and expression of MIB‐1 and p53 immunoreactivity were studied using the avidin‐biotin complex method in three stages of gastric cancer (mucosal, submucosal, deeply invasive cancer, i.e., advanced cancer). The incidence of DNA aneuploidy in intestinal‐type mucosal cancers (15/27, 55.6%) was lower than that of submucosal invasive cancers (14/16, 87.5%) or advanced cancers (19/22, 86.4%), while a low incidence of DNA aneuploidy was observed in each diffuse‐type cancer group (mucosal, 1/12, 8.3%; submucosal invasive, 3/9, 33.3%; advanced, 8/14, 57.1%). Although overexpression of the p53 gene in intestinal‐type cancer was found in early stage, that in diffuse‐type cancer was observed in advanced stage. Among the intestinal‐type mucosal cancers, the MIB‐1 percent positive was higher in aneuploid tumors than diploid ones. DNA aneuploidy and overexpression of the p53 gene may play an important role in the early tumorigenesis of intestinal‐type gastric cancer and in the late event of tumorigenesis of diffuse‐type gastric cancer. Cytometry (Comm. Clin. Cytometry) 38:111–117, 1999. © 1999 Wiley‐Liss, Inc.