Open AccessEvolution of leukocyte immunophenotyping as influenced by the HIV/AIDS pandemic: A short history of the development of gating strategies for CD4 + T‐cell enumeration
Author(s) -
Mandy Francis F.,
Bergeron Michele,
Minkus Tracy
Publication year - 1997
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/(sici)1097-0320(19970815)30:4<157::aid-cyto1>3.0.co;2-h
Subject(s) - immunophenotyping , flow cytometry , immunology , cytometry , gating , biology , pandemic , covid-19 , medicine , neuroscience , pathology , infectious disease (medical specialty) , disease
This brief review of the development of gating strategies for CD4 + T‐cell enumeration is really the story of contemporary clinical flow cytometry. It is the chronicle of its birth, and its slow invasion into the clinical immunology laboratory over the past 15 years. The driving force behind the technological evolution of leukocyte immunophenotyping was, and still is, the impact of the HIV/AIDS pandemic on the discipline of immunology. While, at times, this review of analytical flow cytometry makes metaphorical excursions into the literary world, the objective is not to trivialize technology. Rather, it attempts to illustrate how, in his drive to solve the practical problems of modern medicine, man's essentially eclectic nature excels in an environment of diverse knowledge. This review will examine how technological advancements, such as the introduction of clinical instruments with multi‐color capabilities, and the evolution of leukocyte gating strategies, has influenced the evolution of leukocyte immunophenotyping. Cytometry 30:157–165, 1997. © 1997 Wiley‐Liss, Inc.