Open AccessAssessment of amphotericin B susceptibility in Leishmania infantum promastigotes by flow cytometric membrane potential assay
Author(s) -
Azas N.,
Di Giorgio C.,
Delmas F.,
Gasquet M.,
TimonDavid P.
Publication year - 1997
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/(sici)1097-0320(19970601)28:2<165::aid-cyto10>3.0.co;2-o
Subject(s) - amphotericin b , leishmania infantum , flow cytometry , microbiology and biotechnology , biology , leishmania , antibiotics , parasite hosting , leishmaniasis , antifungal , visceral leishmaniasis , immunology , world wide web , computer science
Flow cytometry was used for measuring the effects of amphotericin B on the membrane of Leishmania infantum strains. The technique was adapted from the rapid flow cytometric membrane potential assay developed by Ordonez and Wehman (Cytometry 22:154–157, 1995) for evaluating antibiotic‐susceptibility of Candida species. The study consisted of measuring membrane potential changes induced by amphotericin B in 3 initial strains and 12 laboratory‐generated variants adapted to grow with amphotericin B. Results showed that, after 3 h of incubation, amphotericin B induced a dose‐related decrease of membrane potential that reached its maximal level at the same concentrations that inhibited parasite growth. These results suggest that the flow cytometric membrane potential assay could be used to assess the susceptibility of Leishmania promastigotes to amphotericin B. Cytometry 28:165–169, 1997. © 1997 Wiley‐Liss, Inc.