Open AccessInduction of tetraploid DNA content by mutant simian virus 40 T antigen that has reduced complex formation with p53
Author(s) -
Lehman John M.,
Albano Perry Mary Beth,
Laffin Judith
Publication year - 1996
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/(sici)1097-0320(19960201)23:2<126::aid-cyto6>3.0.co;2-i
Subject(s) - biology , microbiology and biotechnology , antigen , flow cytometry , virus , population , immunoprecipitation , mutant , dna , virology , cell culture , gene , immunology , genetics , demography , sociology
The 402 mutants (DE, DH, DN) of simian virus (SV) 40 form reduced levels of p53‐T antigen complexes or no complexes in lytically infected cells (CV‐1 cells) relative to wild‐type (wt) virus when assayed by immunoprecipitation. When CV‐1 cells were infected with the 402 mutants, the cells were induced into multiple rounds of DNA synthesis without mitosis, resulting in a large population of cells with >G2 (tetraploid) DNA content similar to wt virus. The levels of T antigen and p53 per cell that were determined by flow cytometry were similar to wt lytically infected cells, with the levels of T antigen increasing as the infection proceeded. The p53 increased as the levels of T antigen increased, similar to a wt infection. These studies demonstrate that, in lytically infected cells with reduced p53‐T antigen complex formation, the cells are induced into multiple rounds of DNA synthesis. © 1996 Wiley‐Liss, Inc.