Open AccessIdentification of a human erythroid progenitor cell population which expresses the CD34 antigen and binds the plant lectin Ulex europaeus I
Author(s) -
Unverzagt Kristen L.,
Martinson Jeffrey,
Lee Wanda,
Stiff Patrick J.,
Williams Stephanie,
Bender James G.
Publication year - 1996
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/(sici)1097-0320(19960101)23:1<54::aid-cyto8>3.0.co;2-m
Subject(s) - cd34 , progenitor cell , identification (biology) , biology , population , antigen , progenitor , cell , microbiology and biotechnology , stem cell , immunology , botany , genetics , medicine , environmental health
Two and three color flow cytometry of normal human bone marrow was used to identify CD34+ progenitor cells and examine their binding to the plant lectin Ulex europaeus I (Ulex). In normal bone marrow, 48.48 ± 17.4% of the CD34+ cells bind to Ulex . Two color flow cytometry was used to sort CD34+ cells, and subsets of CD34+ cells, CD34+ Ulex + and CD34+ Ulex −. These populations were sorted into colony assays to assess myeloid (CFU‐GM) and erythroid (BFU‐E) progenitors. The CD34+ Ulex + subset was 84 ± 14% BFU‐E colonies (mean ± S.D.) and had the highest cloning efficiency of 28 ± 13%. Three color analysis of CD34+ Ulex + cells showed staining with other erythroid (CD71, GlyA) antibodies and lack of staining with myeloid (CD13, CD45RA) antibodies. These studies confirmed the erythroid characteristics of this subpopulation. © 1996 Wiley‐Liss, Inc.