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Solid‐phase combinatorial synthesis using MicroKan reactors, Rf tagging, and directed sorting
Author(s) -
Xiao XiaoYi,
Li Rongshi,
Zhuang Hui,
Ewing Bill,
Karunaratne K.,
Lillig John,
Brown Rick,
Nicolaou K.C.
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(200024)71:1<44::aid-bit7>3.0.co;2-j
Subject(s) - microreactor , modular design , combinatorial synthesis , sorting , solid phase synthesis , computer science , phase (matter) , tracking (education) , reliability (semiconductor) , combinatorial chemistry , process engineering , nanotechnology , chemistry , materials science , engineering , algorithm , physics , operating system , organic chemistry , peptide , psychology , pedagogy , power (physics) , quantum mechanics , catalysis , biochemistry
A modular system for high‐output solid‐phase combinatorial synthesis has been designed and developed. The system employs three technological innovations to achieve its high efficiency and reliability: (1) application of microreactors as the reaction units in solid‐phase synthesis; (2) use of radiofrequency tagging as the non‐chemical tracking method; and (3) development of the directed sorting technology for split & pool synthesis. The system has been successfully applied in the synthesis of compound libraries of several hundred to several thousand compounds in multi‐milligrams per compound quantity by many organizations. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng (Comb Chem) 71:44–50, 2000.