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Use of anion exchange resin‐packed capillary column for rapid detection of anti‐double‐stranded DNA antibody in systemic lupus erythematosus serum
Author(s) -
Lim Taekyu,
Nakamura Noriyuki,
Matsunaga Tadashi
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(20000605)68:5<571::aid-bit12>3.0.co;2-d
Subject(s) - chemistry , antibody , chromatography , dna , capillary action , alkaline phosphatase , isoelectric focusing , isoelectric point , conjugated system , immunoassay , microbiology and biotechnology , biochemistry , immunology , enzyme , biology , materials science , organic chemistry , composite material , polymer
An anti‐double‐stranded (ds) DNA antibodies in serum‐detection system was developed based on flow immunoassay with packed‐capillary column. Alkaline phosphatase conjugated DNA (ALP‐DNA) and anti‐dsDNA antibody were separated from unreacted ALP–DNA on the basis of the difference in isoelectric point. A linear anti‐dsDNA antibody dose‐response curve was obtained between luminescence intensity and concentration of anti‐dsDNA antibody in the range 25–200 IU/mL. This simple technique permits the assay of anti‐dsDNA autoimmune antibodies within 7 minutes. These results give reduced detection times compared with those previously reported (Lim et al., 1999) through the use of capillary columns. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 68: 571–575, 2000.