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Production of medium‐chain‐length polyhydroxyalkanoates by high‐cell‐density cultivation of Pseudomonas putida under phosphorus limitation
Author(s) -
Lee Sang Yup,
Wong Heng Ho,
Choi Jongil,
Lee Seung Hwan,
Lee Sang Cheol,
Han Chul Soo
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(20000520)68:4<466::aid-bit12>3.0.co;2-t
Subject(s) - polyhydroxyalkanoates , pseudomonas putida , phosphorus , fed batch culture , chemistry , food science , nutrient , pseudomonas , carbon source , biochemistry , biology , fermentation , bacteria , organic chemistry , enzyme , genetics
High‐cell‐density fed‐batch cultures of Pseudomonas putida were carried out for the production of medium‐chain‐length polyhydroxyalkanoates (PHAs) using oleic acid as a carbon source. By employing an optimal feeding strategy without the limitation of any nutrient, a high cell concentration of 173 g/L was achieved, but the PHA concentration and PHA content were only 32.3 g/L and 18.7 wt%, respectively. To increase the PHA concentration and content, phosphorus limitation was applied during fed‐bath culture by reducing the initial KH 2 PO 4 concentration. When the initial KH 2 PO 4 concentration was reduced to 4 g/L, cell concentration, PHA concentration, and PHA content obtained in 38 h were 141 g/L, 72.6 g/L, and 51.4 wt%, respectively, resulting in a high productivity of 1.91 g PHA/L per hour. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 68: 466–470, 2000.