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Bacterial cellulose production by Acetobacter xylinum in a 50‐L internal‐loop airlift reactor
Author(s) -
Chao Yaping,
Ishida Takehiko,
Sugano Yasushi,
Shoda Makoto
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(20000505)68:3<345::aid-bit13>3.0.co;2-m
Subject(s) - airlift , pellet , cellulose , continuous stirred tank reactor , suspension (topology) , bioreactor , bacterial cellulose , fermentation , chemistry , chromatography , materials science , food science , composite material , biochemistry , mathematics , organic chemistry , homotopy , pure mathematics
Bacterial cellulose (BC) production was realized in a batch cultivation of Acetobacter xylinum subsp. sucrofermentans BPR2001 in a 50‐L internal‐loop airlift reactor. When the bacterium was cultivated with air supply, 3.8 g/L of BC was produced after 67 hours. When oxygen‐enriched gas was supplied, the concentration of BC was doubled and the production rate of BC was 0.116 g/L · h, which was two times higher than that of air‐supplied culture and comparable to that in a mechanically agitated stirred‐tank fermentor. Bacterial cellulose produced by the airlift reactor formed a unique ellipse pellet (BC pellet), different from the fibrous form which was produced in an agitated stirred‐tank fermentor. The BC‐pellet suspension was demonstrated to have a higher volumetric oxygen transfer coefficient than the fibrous BC suspension in a 50‐L internal‐loop airlift reactor. The mixing time of BC‐pellet suspension in the airlift reactor was also shorter than that in water. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 68: 345–352, 2000.