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Construction of multicomponent catalytic films based on avidin‐biotin technology for the electroenzymatic oxidation of molecular hydrogen
Author(s) -
De Lacey Antonio L.,
Detcheverry Marc,
Moiroux Jacques,
Bourdillon Christian
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(20000405)68:1<1::aid-bit1>3.0.co;2-a
Subject(s) - monolayer , biotinylation , hydrogenase , avidin , chemistry , catalysis , electrode , biotin , viologen , inorganic chemistry , combinatorial chemistry , photochemistry , organic chemistry , biochemistry
Two methods based on the avidin‐biotin technology were developed for the multimonolayer immobilization of Desulfovibrio gigas hydrogenase on glassy carbon or gold electrodes. In both methods the molecular structure of the modified interface was the result of a step‐by‐step process. The first method alternates monolayers of avidin and biotinylated hydrogenase, the mediator (methyl viologen) being free to diffuse in the structure. In the second method, the avidin monolayers were used to immobilize both the biotinylated enzyme and a long‐chain biotinylated viologen derivative. The viologen head of this hydrophilic arm shuttles the electrons between the electrode and the enzyme. The modified electrodes were evaluated for the electroenzymatic oxidation of molecular hydrogen, which has interest for the development of enzymatic fuel cells. The parameters that affect the current density of mediated oxidation of H 2 at the modified electrodes was studied. The second structure, which has given typical catalytic currents of 25 μA per cm 2 for 10 monolayers, was found clearly less efficient than the first structure (500 μA per cm 2 for 10 monolayers). In both methods the catalytic currents increased linearly with the number of monolayers of hydrogenase immobilized, which indicates that the multilayer structures are spatially ordered. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 68: 1–10, 2000.