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Production of poly(3‐hydroxybutyrate‐ co ‐3‐hydroxyhexanoate) by high‐cell‐density cultivation of Aeromonas hydrophila
Author(s) -
Lee Seung Hwan,
Oh Dong Hyun,
Ahn Woo Suk,
Lee Young,
Choi Jongil,
Lee Sang Yup
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(20000120)67:2<240::aid-bit14>3.0.co;2-f
Subject(s) - aeromonas hydrophila , polyhydroxyalkanoates , chemistry , lauric acid , oleic acid , food science , phosphorus , dry weight , bacteria , biochemistry , fatty acid , organic chemistry , botany , biology , genetics
The newly screened Aeromonas hydrophila produces copolymer consisting of 3‐hydroxybutyrate (3HB) and 3‐hydroxyhexanoate (3HHx). The characteristics of cell growth and polymer accumulation were examined using various carbon sources. P(3HB‐ co ‐3HHx) was produced from lauric acid and oleic acid only. P(3HB‐ co ‐3HHx) content can be increased by limitation of phosphorus. A maximal P(3HB‐ co ‐3HHx) content of 28.8 wt% could be obtained in flask culture. By applying the optimally designed nutrient feeding strategy, cell dry weight, P(3HB‐ co ‐3HHx) content, and 3HHx fraction obtained over the course of 43 h were 95.7 g/L, 45.2 wt%, and 17 mol%, respectively, resulting in a productivity of 1.01 g polyhydroxyalkanoate (PHA)/L · h. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 67: 240–244, 2000.