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Potential of an antibacterial ultraviolet‐irradiated nylon film
Author(s) -
Shearer Adrienne E. H.,
Paik James S.,
Hoover Dallas G.,
Haynie Sharon L.,
Kelley Michael J.
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(20000120)67:2<141::aid-bit3>3.0.co;2-#
Subject(s) - irradiation , enterococcus faecalis , escherichia coli , ultraviolet , staphylococcus aureus , population , antimicrobial , chemistry , nuclear chemistry , pseudomonas fluorescens , antibacterial activity , bacteria , food science , materials science , biology , organic chemistry , biochemistry , physics , genetics , optoelectronics , demography , sociology , nuclear physics , gene
The antibacterial effectiveness of an ultraviolet‐irradiated nylon 6,6 film was investigated for potential use as a food‐packaging material to reduce the surface microbial contamination of foods. The film‐surface analyses showed that UV irradiation induced conversion of surface amide groups to amines. Irradiation also increased the dimensional scale of the film surface topography (depth of valleys) approximately 5‐fold on the scale of nanometers. The irradiated nylon demonstrated antagonistic activity against Staphylococcus aureus 25923 and Escherichia coli TV1058 with 4.5 and 6 log reductions, respectively, of an initial population of 10 6 cfu mL −1 . The irradiated nylon was ineffective against Pseudomonas fluorescens 13525 and Enterococcus faecalis 19433 under similar conditions. The film demonstrated increased antimicrobial activity against S. aureus 25923 with increasing temperatures up to 45°C, the highest temperature tested. Protein and salt inhibited the antibacterial nature of the irradiated film. Amines in solution (4.31 × 10 −8 M; the calculated equivalent of amines on the film) killed at least 1 × 10 4 cfu mL −1 E. coli TV1058, and 4.31 × 10 −7 M amines killed up to 1 × 10 7 cfu mL −1 E. coli TV1058. The amines in solution required similar exposure time to the bacteria for population reduction as was observed with the irradiated film. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 67: 141–146, 2000.