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Design and scale up of a process for manganese peroxidase production using the hypersecretory strain Phanerochaete chrysosporium I‐1512
Author(s) -
Herpoël Isabelle,
Asther Marcel,
Sigoillot JeanClaude
Publication year - 1999
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19991120)65:4<468::aid-bit11>3.0.co;2-j
Subject(s) - phanerochaete , manganese peroxidase , bioreactor , chrysosporium , pulp (tooth) , manganese , chemistry , peroxidase , pulp and paper industry , grading scale , biochemistry , enzyme , medicine , organic chemistry , engineering , surgery , pathology
Manganese peroxidase (MnP) production was performed in a airlift bioreactor in which Phanerochaete chrysosporium I‐1512, an MnP hypersecretory strain, was immobilized on a stainless steel mesh. Production was scaled up from a 2.5‐L bench scale to a 100‐L bioreactor. The yield of MnP was increased 2‐fold and reached 6600 U L −1 . These results indicate the feasibility of MnP production on a medium scale, which promises sufficient MnP availability for its use in pulp bleaching at industrial scale. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 65: 468–473, 1999.