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Chiral compounds from bacterial polyesters: Sugars to plastics to fine chemicals
Author(s) -
Lee Sang Yup,
Lee Young,
Wang Fulai
Publication year - 1999
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19991105)65:3<363::aid-bit15>3.0.co;2-1
Subject(s) - depolymerization , polyhydroxybutyrate , polyhydroxyalkanoates , polyester , cupriavidus necator , chemistry , yield (engineering) , organic chemistry , in vivo , biochemistry , bacteria , materials science , biology , genetics , microbiology and biotechnology , metallurgy
A novel and efficient method for the production of enantiomerically pure ( R )‐(‐)‐hydroxycarboxylic acids by in vivo depolymerization of microbial polyester polyhydroxyalkanoates (PHAs) was developed. Using this method, several model compounds, ( R )‐(‐)‐3‐hydroxyalkanoic acids, consisting of 4 to 12 carbon atoms, and ( R )‐(‐)‐3‐hydroxy‐5‐phenylvaleric acid, could be prepared. In particular, ( R )‐(‐)‐3‐hydroxybutyric acid could be efficiently prepared by this method. By providing the environmental condition in which cells possess high activity of intracellular PHA depolymerase and low activity of ( R )‐(‐)‐3‐hydroxybutyric acid dehydrogenase, ( R )‐(‐)‐3‐hydroxybutyric acid could be produced with a yield of 96% in only 30 min by in vivo depolymerization of polyhydroxybutyrate (PHB) accumulated in Alcaligenes latus . © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 65: 363–368, 1999.