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Effect of harvesting protocol on performance of a hollow fiber bioreactor
Author(s) -
Gramer Michael J.,
Poeschl Douglas M.,
Conroy Mark J.,
Hammer Bruce E.
Publication year - 1999
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19991105)65:3<334::aid-bit11>3.0.co;2-l
Subject(s) - bioreactor , fiber , protocol (science) , biochemical engineering , pulp and paper industry , chemistry , materials science , biology , engineering , botany , composite material , medicine , alternative medicine , pathology
In this study, a bioreactor subject to Starling flow in closed shell batch harvest mode was compared to two forms of additional forced extracapillary (EC) space convection including EC circulation and EC cycling. Despite the presence of Starling flow as the dominant EC convection mechanism in the batch harvest system, the bioreactor start up was fairly good. However, the antibody productivity of the batch harvest system fell off rapidly after day 20 resulting in only 4.5 g of antibody produced. EC circulation with flow parallel to the fibers had a slightly better start up than the batch harvest. However, the antibody productivity also dropped after day 20 with EC circulation, resulting in only 7.5 g of antibody produced. EC cycling with flow both parallel and perpendicular to the fibers resulted in a start up similar to that of EC circulation. However, in contrast to the other two systems, antibody productivity in the EC cycling system was stable over the 60‐day experiment resulting in the production of 23 g of antibody. These results demonstrate the importance of inducing the proper flow distribution in the EC space to allow consistent and stable production in hollow fiber bioreactors. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 65: 334–340, 1999.