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Problems in predicting cell damage from bubble bursting
Author(s) -
Dey Dipankar,
Emery A. Nicholas
Publication year - 1999
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19991020)65:2<240::aid-bit16>3.0.co;2-p
Subject(s) - bursting , bubble , mechanics , biological system , biology , neuroscience , physics
The question is addressed as to whether observed parameter(s) characterizing single bubble burst (bubble jet height and speed) can be used to predict cell damage in sparged animal cell cultures. Bubble burst profiles are examined in the presence of realistic concentrations of fetal calf serum (FCS) or Pluronic F‐68 using a high‐speed video technique. The damage to TBC3 hybridoma cells from bubble sparging, characterized as a first‐order decline, is reduced by even very small concentrations of both FCS and Pluronic F‐68, but neither single bubble burst parameters nor surface properties give useable correlations with death rate constants. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 65: 240–245, 1999.