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Construction of a multi RE module: Exploitation of mechanochemistry of restriction endonucleases
Author(s) -
Agarwal Pratul K.,
Bhattacharya Sanjoy K.
Publication year - 1999
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19991020)65:2<233::aid-bit15>3.0.co;2-s
Subject(s) - mechanochemistry , restriction enzyme , computational biology , chemistry , biochemical engineering , biology , engineering , dna , nanotechnology , biochemistry , materials science
We describe the construction of a multi‐immobilized restriction endonuclease module (Multi RE module). We demonstrate that the applied mechanical stress enables modulation of enzyme activity and modulation of recognition site selectivity (in oligonucleotides of ∼200 bp) of immobilized restriction endonucleases. The central module which is consisted of different strips of immobilized restriction endonucleases allows limited digestion of a large DNA sample in a controlled manner as a function of applied mechanical stress on strips. The stress–activity relationship and the effect of repeated cycles of stress and relaxation on the immobilized strips are presented here. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 65: 233–239, 1999.