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GFP‐expressing mammalian cells for fast, sensitive, noninvasive cell growth assessment in a kinetic mode
Author(s) -
Hunt L.,
Jordan M.,
De Jesus M.,
Wurm F. M.
Publication year - 1999
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19991020)65:2<201::aid-bit10>3.0.co;2-h
Subject(s) - fluorometer , green fluorescent protein , fluorescence , cell culture , cell growth , cell , recombinant dna , biology , growth rate , chinese hamster ovary cell , biophysics , microbiology and biotechnology , chemistry , chromatography , biochemistry , genetics , gene , physics , geometry , mathematics , quantum mechanics
This study correlates the fluorescent signal from stable recombinant CHO cell lines expressing the green fluorescent protein (GFP) at high levels with biomass or cell number, extending the use of fluorescent proteins to applications and assays where cell growth rates are important. Using a standard fluorometer, growth of these cells can be quantified noninvasively in multiwell plates, and because signals are obtained without preparation, the same culture samples can be measured repeatedly. Even with a small relative change in biomass, the specific growth rate can be determined in a few hours. The dynamics of cell populations can now be studied with high sensitivity, low error rate, and minimum sample preparation. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 65: 201–205, 1999.