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Independent prediction of naphthalene transport and biodegradation in soil with a mathematical model
Author(s) -
Ahn IkSung,
Ghiorse William C.,
Lion Leonard W.,
Shuler Michael L.
Publication year - 1999
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19991005)65:1<65::aid-bit8>3.0.co;2-k
Subject(s) - naphthalene , biodegradation , pseudomonas putida , chemistry , substrate (aquarium) , chromatography , biological system , chemical engineering , organic chemistry , engineering , ecology , biology , enzyme
Experiments were performed to test the ability of a mathematical model to predict naphthalene transport and biodegradation. Pseudomonas putida G7, a model bacterial strain capable of degrading naphthalene, was added to a column packed with the soil that had been pre‐equilibrated with naphthalene. Model prediction for transport and degradation were based on predetermined parameters that described naphthalene desorption kinetics and the utilization of naphthalene by the test bacterium. However, initial prediction for naphthalene biodegradation was high, and the formation of cell aggregates is advanced as a plausible explanation. Access of substrate to cells in the interior of an aggregate would be restricted. When the numerical simulation was conducted with a factor to account for cell aggregation, it successfully described the experimental data. Thus, with a single adjustable parameter (an average effectiveness factor), the model predicted macroscopic responses of naphthalene in soil‐columns where naphthalene was subject to transport and biodegradation. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 65: 65–75, 1999.