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Estimation of P‐to‐O ratio in Bacillus subtilis and its influence on maximum riboflavin yield
Author(s) -
Sauer Uwe,
Bailey James E.
Publication year - 1999
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19990920)64:6<750::aid-bit15>3.0.co;2-s
Subject(s) - riboflavin , bacillus subtilis , oxidative phosphorylation , yield (engineering) , chemistry , pyrazine , biochemistry , stoichiometry , electron transport chain , biophysics , bacteria , biology , stereochemistry , thermodynamics , organic chemistry , genetics , physics
Abstract Simultaneous growth and riboflavin overproduction were investigated using a previously developed stoichiometric model of Bacillus subtilis metabolism. A fit of model predictions to experimental data was used to obtain estimates of fundamental energetic parameters of B. subtilis. Although multiple solutions describe the experimental data, evidence for a P‐to‐O ratio of about 1⅓ mole of ATP produced per atom of oxygen consumed in oxidative phosphorylation was provided by genomic analysis of electron transport components, because no homologue of the proton‐translocating NADH dehydrogenase I was found in the B. subtilis genome database. These results allow us to devise a rational metabolic engineering strategy to improve riboflavin production. The potential influence of increased energy coupling in oxidative phosphorylation on riboflavin yield is discussed. Higher coupling is most significant under carbon‐limiting conditions in slow‐growing cells, that is, in fed‐batch processes of industrial interest. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 64: 750–754, 1999.