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Isolation of a novel microorganism, Pestalotia heterocornis, producing paclitaxel
Author(s) -
Noh MoonJong,
Yang JaeGwon,
Kim KyungSoo,
Yoon YoungMean,
Kang KyoungAe,
Han HeeYong,
Shim SungBo,
Park HoJin
Publication year - 1999
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19990905)64:5<620::aid-bit13>3.0.co;2-w
Subject(s) - paclitaxel , yield (engineering) , fermentation , chemistry , mass spectrometry , isolation (microbiology) , chromatography , food science , biology , microbiology and biotechnology , materials science , chemotherapy , genetics , metallurgy
Pestalotia heterocornis was isolated from soil collected in yew forest and was shown to produce paclitaxel in semisynthetic liquid media. The presence of paclitaxel in the fungal extract was confirmed by FAB mass spectrometry and NMR spectroscopy. The maximum yield of paclitaxel was 31 μg per liter. Optimal paclitaxel production occurred after 5–7 days in a 20‐liter scale fermentation at 23°C. These results indicate that P. heterocornis is an excellent candidate for consideration in fermentation technology. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 64: 620–623, 1999.